Zebra mussels (Dreissena polymorpha) are invasive species that alter ecosystems and food webs with the potential to affect aquatic mercury cycling and bioaccumulation in fishes, although the effect of zebra mussels on fish tissue mercury has not been tested in inland lakes. We assessed differences in fish tissue mercury concentrations and food webs in Minnesota lakes with and without zebra mussels while controlling for other lake and watershed characteristics. Mercury concentrations in adult walleye (Sander vitreus) and yellow perch (Perca flavescens) were 72 % and 157 % higher, respectively, in lakes containing zebra mussels compared to uninvaded lakes. Mercury in young of year (age-0) fish was also elevated, with mercury concentrations 97 % and 82 % higher in age-0 walleye and yellow perch, respectively, in zebra mussel lakes. Walleye mercury concentrations exceeded 0.22 ppm — a threshold triggering more restrictive human consumption advisories for sensitive populations — at a 23 % smaller size, and average-sized walleye (420 mm) exceeded this threshold at a rate of 77 % in invaded lakes, compared to 35 % in uninvaded lakes. Walleye and yellow perch relied more on littoral resources in lakes with zebra mussels but did not feed at meaningfully higher trophic levels. Increased fish tissue mercury in lakes invaded by zebra mussels have consequential implications for fisheries and human health.