Development of a large-volume concentration method to recover infectious avian influenza virus from the aquatic environment
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Abstract
Since late 2021, outbreaks of highly pathogenic avian influenza virus have caused a record number of mortalities in wild birds, domestic poultry, and mammals in North America. Wetlands are plausible environmental reservoirs of avian influenza virus; however, the transmission and persistence of the virus in the aquatic environment are poorly understood. To explore environmental contamination with the avian influenza virus, a large-volume concentration method for detecting infectious avian influenza virus in waterbodies was developed. A variety of filtering, elution, and concentration methods were explored, in addition to testing filtering speeds using artificially amended 20 L water matrices (deionized water with sterile dust, autoclaved wetland water, and wetland water). The optimal protocol was dead-end ultrafiltration coupled with salt solution elution and centrifugation concentration. Using this method, infectious virus was recovered at 1 × 10−1 50% egg infectious dose per milliliter (EID50/mL), whereas viral RNA was detected inconsistently down to 1 × 100 EID50/mL. This method will aid in furthering our understanding of the avian influenza virus in the environment and may be applicable to the environmental detection of other enveloped viruses.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Development of a large-volume concentration method to recover infectious avian influenza virus from the aquatic environment |
| Series title | Viruses |
| DOI | 10.3390/v16121898 |
| Volume | 16 |
| Issue | 12 |
| Year Published | 2024 |
| Language | English |
| Publisher | MDPI |
| Contributing office(s) | Upper Midwest Water Science Center |
| Description | 1898, 14 p. |
| Country | United States |
| State | Ohio |
| City | Columbus |
| Google Analytic Metrics | Metrics page |