Fish behavior after passage or transfer around dams is a critical component in determining whether the goals of these efforts are achieved, but these behaviors are often poorly understood. An elevator was constructed in the lowermost hydroelectric dam on the Menominee River, Wisconsin–Michigan; it is the first elevator specifically designed to capture Lake Sturgeon Acipenser fulvescens for upstream transfer above two dams, providing access to high-quality spawning and early life habitat. Our objectives were to determine whether (1) Lake Sturgeon transferred upstream remained upstream for at least one spawning opportunity; (2) spawning opportunity, time to reach the next dam upstream, and residency in different segments of the river were related to sex, capture method (elevator versus electrofishing), and season of transfer; and (3) the probability of fish transitioning back downstream of the two dams varied among months. We evaluated posttransfer behaviors of 139 Lake Sturgeon that were captured in the elevator or by electrofishing, implanted with acoustic transmitters, transferred upstream (in spring or fall) from fall 2014 to spring 2017, and monitored until fall 2018 using 20–23 stationary acoustic receivers deployed throughout the river. Most Lake Sturgeon (91%) remained upstream for at least one spawning opportunity. The probability of remaining for one spawning opportunity was not related to sex, fish capture method, or season of transfer. Residency times within the two impoundments and time to reach the next dam upstream varied among individual fish. A multistate model indicated that monthly survival after upstream transfer was high and that Lake Sturgeon typically remained above both dams in late fall to early spring, with most downstream movements occurring in April and May. Our results indicate that Lake Sturgeon transferred upstream have the potential to contribute offspring that may help to bolster the Lake Sturgeon population in Lake Michigan, but additional research may help in determining whether these contributions occur.