Anadromous fishes play important roles in nutrient dynamics for freshwater ecosystems; however, the trophic pathways have been less documented for iteroparous species like river herring (Alosa pseudoharengus and A. aestivalis) compared to semelparous species like Pacific salmon (Oncorhynchus spp.). Given recent increases in restoration activities to improve connectivity, an understanding of how anadromous river herring influence the morphology, physiology, and life history of predatory fishes can help predict restoration responses. We aimed to quantify the trophic influence of juvenile anadromous river herring on predatory white perch (Morone americana) and yellow perch (Perca flavescens) using a combination of stable isotopes, growth rates, and condition indices. We sampled six lakes in coastal Massachusetts—three lakes with anadromous river herring and three similar lakes without river herring. Bayesian mixing models of δ¹³C and δ¹⁵N indicated white perch consumed juvenile river herring in higher proportions (69–75%) compared to co-occurring prey fishes (11–16%). Lakes with juvenile river herring contained perch with significantly higher condition values, higher immature growth rates (age 1 and 2), lower mature growth rates (> age 3), significantly smaller mature lengths, and lower mortality rates compared to perch in lakes without river herring. These divergent life history traits of perch in response to consumption of juvenile river herring are consistent with observations in other predatory fishes. Direct links between river herring and predator condition, growth, and life history trajectories suggest broad influences on ecosystem structure across trophic levels through physiological, morphometric, and life history modifications.