There is a need to prioritize research and data collection in river basins by integrating information from environmental, ecological, and socioeconomic datasets to maintain acceptable water quality for human uses and ecosystem health. Multiple anthropogenic and natural stressors are responsible for driving changes in stream temperatures that can alter ecosystems and degrade water quality. These stressors are variable spatially and temporally, which can be challenging for prioritizing monitoring and research. In this study, an evaluation of variables related to stream temperature was performed for 163 candidate basins of the contiguous United States to highlight potential focal areas. Thirty variables were combined in six components: anthropogenic forcing, natural forcing, climate change, ecological sensitivity, socioeconomic sensitivity, and data availability. The components were incorporated into three themes: vulnerability of streams to thermal change, vulnerability of ecosystems, and vulnerability of communities. By evaluating each theme separately, patterns of vulnerability and potential resiliency were identified as well as consistency in ranks within the themes. For example, results of the national scale ranking indicated the Southern California Coastal basin (within California-Nevada region) was the highest-ranking priority in vulnerability of streams to thermal change and vulnerability of ecosystems. The analysis also identified vulnerable basins with gaps in monitoring. For example, the Missouri-Oahe basin (within the Northern High Plains region) was the highest-ranking priority for vulnerable communities with limitations in data availability. The ranking approach provides insight into basins that are resilient and are ideal candidates for monitoring and research.