In February 2020, we held a workshop where we sought to identify where states should reintroduce or augment brook floater to minimize the probability of extinction within a state. We focused on Massachusetts and Connecticut, two states with only a few, small populations still extant, that likely need population restoration to prevent statewide extirpation. We identified that restoration actions aimed at redundancy (number of populations), representation (number of occupied basins), and resiliency (population size) were constrained by resource availability such as limited broodstock , staff time, and budgets. Optimal restoration locations depended on habitat conditions, the status (viability) of nearby mussel populations, population size (number of individuals), and the location within watersheds; all important considerations in addressing population persistence. Restoration actions also accounted for the risk of disease transmission among mussels and fish, and the genetic health and diversity of mussel populations. The workshop identified the multiple, compounding uncertainties related to population restoration, identified information gaps critical to decision making, and charted a path forward to make decisions given uncertainties. The optimization approach developed can be used to select specific watersheds for restoration in any state, province, or region and can easily be adapted as new information becomes available.