This report presents results from a four-year project (2018–2022) to document the effects of small, run-of-river dams and dam removal on water quality (stream temperature and dissolved oxygen (DO)), aquatic macroinvertebrates, and fishes. Temperature and DO are critical water quality parameters that shape biogeochemical processes and biotic assemblages in streams. Macroinvertebrate and fish assemblages can be reflective of habitat and water quality due to their diversity and sensitivity to high temperatures and low DO and are often used as indicators of ecosystem health (e.g., Clean Water Act Section 401). This study aimed to better explain the responses of these important ecological parameters to small dam removals, which may support a more comprehensive understanding of the benefits of restoration to aquatic ecosystems.

We collected pre- and post-restoration water quality data and macroinvertebrate samples at 16 small dams in Massachusetts that have been removed (10 sites) or are currently being considered for removal (6 sites). General results from these monitoring efforts indicate that:

● 15 of 16 small dams increased impoundment water temperatures and warming persisted downstream at 11 of those sites, relative to upstream. Dam removal reduced summer impoundment warming at 7 of 10 removal sites and reduced downstream warming at 5 of 10 sites. These in-stream temperature improvements occurred within 5 years after dam removal.

● 13 of 16 small dams negatively impacted dissolved oxygen (DO) concentrations within the impoundments, but the magnitude of impact varied across sites. Negative impoundment DO impacts did not consistently translate downstream, and downstream responses to dam removal were generally minimal and variable across sites. Dam removal significantly reduced negative impoundment DO impacts within 1 year after removal at 7 of 10 sites, and sites with greater pre-removal impacts experienced the greatest magnitude of DO recovery after dam removal.

● Interannual variability in dam impacts on water quality across sites suggests periods of extreme weather (i.e., droughts or high precipitation) due to climate change may exacerbate adverse impacts from run-of-river dams.

● Macroinvertebrate assemblages within dam impoundments differed from assemblages in adjacent un-impounded stream sections and exhibited a loss of sensitive organisms (an average of 17% fewer). Dam removal led to more similar macroinvertebrate assemblages throughout most stream sections, and recovery of sensitive taxa occurred relatively quickly (1-3 years).

● Fish species richness increased upstream at 2 of 10 removal sites, suggesting potential increases in fish passage from downstream reaches. However, particular species, such as American Eel (Anguilla rostrata), exhibited both positive and negative responses to dam removal across study sites. Incorporating more sites with pre-and post-dam removal fish data could allow for better understanding factors explaining site-specific differences.