Characterizing traffic-related air pollutants (TRAPs), which significantly impact health, and greenhouse gases (GHGs) can be challenging in urban environments. Mobile monitoring has the potential to capture the spatial distribution of these pollutants. We present results from a campaign using the Denver Mobile Monitoring Laboratory (DMML) in the summer of 2023 when we measured ultrafine particles (UFPs), black carbon (BC), ozone (O₃), methane (CH₄)_, and carbon dioxide (CO₂) concentrations in Denver, CO. Despite our campaign being brief, we obtained several interesting results. We observed elevated UFP and BC concentrations on major roads. In contrast, O₃ concentrations were higher on neighborhood streets and roads and in the industrial neighborhood of Commerce City. We consistently observed elevated CH₄ concentrations (>2.5 ppm) on highway I-70, suggesting the presence of a previously unknown major source of CH₄. The CH₄ concentrations measured in our campaign did not align with those from an overlapping aerial campaign, suggesting that mobile monitoring is crucial to capture important, potentially intermittent CH₄ hotspots in cities. We evaluated if trees mitigated pollution concentrations, as planting trees is a key policy initiative of the city of Denver. We observed significant negative associations between tree canopy coverage and UFPs, BC, and CH₄, and a positive association with O₃ when using linear mixed-effects regression models. Our work highlights the importance of investigating the role of tree canopy coverage to mitigate TRAPs.