Mountains comprise ∼30% of the Earth's surface, but mercury (Hg) cycling in these regions remains understudied, particularly in the semi-arid western U.S. where strong climatic and ecological gradients in mountainous landscapes influence Hg deposition, retention, and bioaccumulation. In this study, we quantified growing season inputs, storage, and bioaccumulation of Hg along a ∼2,000 m elevation gradient in the Colorado Rocky Mountains, spanning the plains to the alpine. We measured Hg in atmospheric deposition, vegetation, soil, and 12-day-old chickadees. Accounting for percent canopy cover, open precipitation was the largest component of atmospheric deposition at all elevations, followed by throughfall and litterfall fluxes. Atmospheric Hg fluxes peaked at mid-elevations, likely due to cloud-cap dynamics and denser canopy cover. Total gaseous Hg and precipitation fluxes were highest at low elevations, likely reflecting local emissions and meteorological pooling. Surface soil Hg storage was more strongly predicted by organic matter content (R² = 0.49; p < 0.01) and water retention (R² = 0.45; p < 0.01) than by elevation (R² = 0.21; p < 0.05). Alpine soils (66.3 ± 25.3 ng g⁻¹) had significantly higher total Hg concentrations than lower elevations (<41.0 ± 12.7 ng g⁻¹; p < 0.01), likely reflecting slower organic matter turnover. Soils on north-facing slopes also retained significantly higher pools of Hg in surface soils compared with south- and east-facing slopes. Vegetation Hg pools were greatest in the alpine region, likely due to long-lived plant species. Methylmercury (MeHg) concentrations in chickadee feathers peaked at mid-elevations (205 ± 155 ng g⁻¹), corresponding to higher ecosystem Hg inputs via throughfall. Our results show that deposition, canopy cover, and meteorological conditions—not elevation alone—predict Hg retention and bioaccumulation.