The Marfa Basin in West Texas is a late Palaeozoic synorogenic depocenter associated with regional deformation linked to the Ancestral Rocky Mountains (ARM) and Ouachita–Marathon–Sonora (OMS) orogenies in southwestern Laurentia. Basin strata range in age from Middle Pennsylvanian to the middle Permian and include the Cieneguita, Alta, Pinto Canyon, Rose Mine and Mina Grande Formations. Sandstone petrography and detrital zircon (DZ) U–Pb and (U–Th)/He double dating data from these strata reveal three tectonically driven sedimentation stages: syntectonic ARM deposition, progressive OMS foredeep deposition and an orogenic transition. The Cieneguita and lower part of the Alta Formations exhibit a Mesoproterozoic DZ age signature (~1318 and ~1076 Ma age peaks) and quartzo-feldspathic sandstone compositions sourced from the adjacent ARM-related Diablo Platform basement uplift in the Middle Pennsylvanian to earliest Permian. In contrast, the upper part of the Alta Formation, as well as the Pinto Canyon and Rose Mine Formations, have peri-Gondwanan DZ age signatures, with Mesoproterozoic (~1069–1036 Ma age peaks), Neoproterozoic–Cambrian (~700–490 Ma) and Palaeozoic (~490–300 Ma) age modes and litho-quartzose sandstone compositions derived from the OMS fold-and-thrust belt and orogenic hinterland during the early to middle Permian. The lower to middle parts of the Alta Formation have alternating DZ age signatures and sandstone compositions from both ARM and OMS sources, revealing that the transition in the sediment supply occurred during the middle Wolfcampian. This transition was not characterised by source mixing, but rather by sediment interfingering alternately sourced from the Diablo Platform uplift and the advancing OMS belt. These observations are confirmed by the DZ He ages, which reveal distinct cooling histories for both source terranes. These results document a switch from ARM- to OMS-related syntectonic deposition in southwestern Laurentia during the early Permian, demonstrating that ARM-driven deformation largely preceded the continental collision along the Marathon segment of the OMS orogen.