Since 2003, the U.S. Geological Survey, in cooperation with the Sonoma County Water Agency, has been collecting chemical, microbiological, and isotopic data from surface-water and groundwater sites in Mendocino and Sonoma Counties, California. The investigation is being conducted to determine water-quality baseline conditions for the Russian River during the summer months and to characterize the water-quality in the area of the Sonoma County Water Agency's water-supply facility where Russian River water is diverted and treated by riverbank filtration. This report is a compilation of the hydrologic and water-quality data collected from 14 Russian River sites, 8 tributary sites, 1 gravel-terrace pit site, 14 groundwater wells, and a wastewater treatment plant between the city of Ukiah and the town of Duncans Mills for the period August 2005 through October 2010. Field measurements included discharge, barometric pressure, dissolved oxygen, pH, specific conductance, temperature, and turbidity. All samples were analyzed for nutrients, major ions, trace metals, total and dissolved organic carbon, organic wastewater compounds, standard bacterial indicators, and the stable isotopes of hydrogen and oxygen. Standard bacterial indicators included total coliform, Escherichia coli, enterococci, and Clostridium perfringens for the period 2005 through 2007, and total and fecal coliform, and enterococci for 2010. In addition, enrichment of enterococci was performed on all surface-water samples collected during summer 2006, for detection of the human-associated enterococcal surface protein in Enterococcus faecium to assess the presence of sewage effluent in the Russian River. Other analyses included organic wastewater compounds of bed sediment samples collected from four Russian River sites during 2005; carbon-13 isotopic values of the dissolved inorganic carbon for surface-water and groundwater samples collected during 2006; human-use pharmaceuticals on Russian River samples collected during 2007 and 2010; and the radiogenic isotopes tritium and carbon-14 for groundwater samples collected during 2008.