From 2002 through 2006, the U.S. Geological Survey National Water-Quality Assessment Program conducted studies investigating mercury biogeochemistry and food-web bioaccumulation in eight streams from three distinct geographic areas of the United States. These streams varied greatly in environmental characteristics, including land-cover, hydrologic, climatic, and chemical characteristics. They ranged from a clear-water, high-gradient, low-percentage wetland stream in the Cascade Mountains of Oregon, to an urban stream near Milwaukee, Wisconsin, to a low-gradient, blackwater stream draining the Okefenokee and Pinhook Swamps along the Georgia-Florida border. This report summarizes the environmental settings of these eight streams.