Comparison of two cell lysis procedures for recovery of microcystins in water samples from silver lake in Dover, Delaware, with microcystin producing cyanobacterial accumulations
Open-File Report
2008-1341
Prepared in collaboration with Abraxis, LLC, Delaware Department of Natural Resources and Environmental Control Division of Water Resources Environmental Laboratory, and the University of Delaware
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Abstract
A collaboration was developed between Abraxis, LLC, the State of Delaware Department of Natural Resources and Environmental Control Division of Water Resources Environmental Laboratory, the University of Delaware, and the United States Geological Survey to investigate the efficacy of the QuikLyse procedure developed by Abraxis, LLC as an alternative cell-lysis technique suitable for use with an existing liquid chromatography/tandem mass spectrometry research method developed at the United States Geological Survey Organic Geochemistry Research Laboratory to analyze cyanotoxins. A comparison of three sequential freeze/thaw cycles versus QuikLyse, a proprietary chemical lysis procedure was conducted on four water samples collected from Silver Lake in Dover, Delaware. Results from the Abraxis Microcystins-DM enzyme-linked immunosorbent assay and liquid chromatography/tandem mass spectrometry were tabulated as a function of the cell lysis technique. Stastical comparison of percent relative standard deviations showed no significant difference (alpha = 0.05) between both cell-lysis techniques when measured by enzyme-linked immunosorbent assay or liquid chromatography/tandem mass spectrometry for three of the four samples.
Suggested Citation
Loftin, K.A., Meyer, M.T., Rubio, F., Kamp, L., Humphries, E., and Whereat, E., 2008, Comparison of two cell lysis procedures for recovery of microcystins in water samples from silver lake in Dover, Delaware, with microcystin producing cyanobacterial accumulations (Version 1.0): U.S. Geological Survey Open-File Report 2008-1341, vi, 10 p., https://doi.org/10.3133/ofr20081341.
ISSN: 2331-1258 (online)
Study Area
| Publication type | Report |
|---|---|
| Publication Subtype | USGS Numbered Series |
| Title | Comparison of two cell lysis procedures for recovery of microcystins in water samples from silver lake in Dover, Delaware, with microcystin producing cyanobacterial accumulations |
| Series title | Open-File Report |
| Series number | 2008-1341 |
| DOI | 10.3133/ofr20081341 |
| Edition | Version 1.0 |
| Year Published | 2008 |
| Language | English |
| Publisher | U.S. Geological Survey |
| Publisher location | Reston, VA |
| Contributing office(s) | Kansas Water Science Center, Toxic Substances Hydrology Program |
| Description | vi, 10 p. |
| Country | United States |
| State | Delaware |
| County | Kent County |
| City | Dover |