Water, bed sediment, and biota were sampled in streams from Butte to below Missoula as part of a program to characterize aquatic resources in the upper Clark Fork basin of western Montana. Sampling stations were located on the Clark Fork and major tributaries. Water-quality data were obtained periodically at 16 stations during October 1994 through September 1995 (water year 1995). Data for twelve bed-sediment and eleven biological stations were obtained in August 1995. The primary constituents analyzed were trace elements associated with mine tailings from historical mining and smelting activities. Water-quality data include concentrations of major ions, trace element, and suspended sediment in stream samples collected periodically during water year 1995. Daily values of streamflow, suspended- sediment concentrations in the fine-grained and bulk fractions. Biological data include trace- element concentrations in whole-body tissue of aquatic benthic insects. Quality-assurance data are reported for analytical results of water, bed sediment, and biota. Statistical summaries of water-quality, bed-sediment, and biological data are provided for the period of record at each station since 1985.