This study is part of a larger effort to identify fresh water needs throughout the region and develop a mechanism for basinwide water management. Quantitative estimates of the amount of aquatic habitat in the floodplain in relation to river flow are presented. Plates show streams, lakes, and floodplain forests connected to the main river channel at selected flows; an analysis of long-term flow record in the Apalachicola River; and a review of the literature regarding fishes in floodplains of the Apalachicola River and other rivers of the Eastern United States. Examples show how this report can be used to assess impacts of flow alterations on aquatic habitats and fishes.