Preparative free-flow electrophoresis as a method of fractionation of natural organic materials
Water Supply Paper
1817-D
By: J.A. Leenheer and Ronald L. Malcolm
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Abstract
Preparative free-flow electrophoresis was found to be an efficient method of conducting large-scale fractionations of the natural organic polyelectrolytes occurring in many surface waters and soils. The method of free-flow electrophoresis obviates, the problem of adsorption upon a supporting medium and permits the use of high potential gradients and currents because of an efficient cooling system. Separations were monitored by determining organic carbon concentration with a dissolved carbon analyzer, and color was measured by absorbance at 400 nanometers. Organic materials from waters and soils were purified by filtration, hydrogen exchange, and dialysis and were concentrated by freeze drying or freeze concentration. In electrophoretic fractionations of natural organic materials typically found in surface waters and soils, color was found to increase with the charge of the fraction.
Suggested Citation
Leenheer, J., and Malcolm, R., 1973, Preparative free-flow electrophoresis as a method of fractionation of natural organic materials: U.S. Geological Survey Water Supply Paper 1817, iii, 14 p. :illus. ;24 cm., https://doi.org/10.3133/wsp1817D.
| Publication type | Report |
|---|---|
| Publication Subtype | USGS Numbered Series |
| Title | Preparative free-flow electrophoresis as a method of fractionation of natural organic materials |
| Series title | Water Supply Paper |
| Series number | 1817 |
| Chapter | D |
| DOI | 10.3133/wsp1817D |
| Year Published | 1973 |
| Language | ENGLISH |
| Publisher | U.S. Govt. Print. Off., |
| Description | iii, 14 p. :illus. ;24 cm. |