Field detection of avian influenza virus in wild birds: evaluation of a portable rRT-PCR system and freeze-dried reagents
John Y. Takekawa, Samuel A. Iverson, Annie K. Schultz, Nichola J. Hill, Carol J. Cardona, Walter M. Boyce, Joseph P. Dudley
2010, Journal of Virological Methods (166) 92-97
Wild birds have been implicated in the spread of highly pathogenic avian influenza (HPAIV) of the H5N1 subtype, prompting surveillance along migratory flyways. Sampling of wild birds is often conducted in remote regions, but results are often delayed because of limited local analytical capabilities, difficulties with sample transportation and permitting,...
Characterization of recombinant Raccoonpox Vaccine Vectors in Chickens
S.-H. Hwa, Keith P. Iams, Jeffrey S. Hall, B.A. Kingstad, Jorge E. Osorio
2010, Avian Diseases (54) 1157-1165
Raccoonpox virus (RCN) has been used as a recombinant vector against several mammalian pathogens but has not been tested in birds. The replication of RCN in chick embryo fibroblasts (CEFs) and chickens was studied with the use of highly pathogenic avian influenza virus H5N1 hemagglutinin (HA) as a model antigen...
Rice production systems and avian influenza: Interactions between mixed-farming systems, poultry and wild birds
S.B. Muzaffar, John Y. Takekawa, D.J. Prosser, S. H. Newman, X. Xiao
2010, Waterbirds (33) 219-230
Wild waterfowl are the reservoir for avian influenza viruses (AIVs), a family of RNA viruses that may cause mild sickness in waterbirds. Emergence of H5N1, a highly pathogenic avian influenza (HPAI) strain, causing severe disease and mortality in wild birds, poultry and humans, had raised concerns about the role of...
Waterfowl ecology and avian influenza in california: Do host traits inform us about viral occurrence?
N.J. Hill, John Y. Takekawa, C.J. Cardona, Joshua T. Ackerman, A.K. Schultz, K.A. Spragens, W.M. Boyce
2010, Avian Diseases (54) 426-432
We examined whether host traits influenced the occurrence of avian influenza virus (AIV) in Anatidae (ducks, geese, swans) at wintering sites in California's Central Valley. In total, 3487 individuals were sampled at Sacramento National Wildlife Refuge and Conaway Ranch Duck Club during the hunting season of 2007-08. Of the 19...
Limited evidence of trans-hemispheric movement of avian influenza viruses among contemporary North American shorebird isolates
John M. Pearce, Andrew M. Ramey, S. Ip, Robert E. Gill Jr.
2010, Virus Research (148) 44-50
Migratory routes of gulls, terns, and shorebirds (Charadriiformes) are known to cross hemispheric boundaries and intersect with outbreak areas of highly pathogenic avian influenza (HPAI). Prior assessments of low pathogenic avian influenza (LPAI) among species of this taxonomic order found some evidence for trans-hemispheric movement of virus genes. To specifically...
Transmission and reassortment of avian influenza viruses at the Asian-North American interface
Andrew M. Ramey, John M. Pearce, Craig R. Ely, Lisa M. Sheffield Guy, David B. Irons, Dirk V. Derksen, S. Ip
2010, Virology (406) 352-359
Twenty avian influenza viruses were isolated from seven wild migratory bird species sampled at St. Lawrence Island, Alaska. We tested predictions based on previous phylogenetic analyses of avian influenza viruses that support spatially dependent trans-hemispheric gene flow and frequent interspecies transmission at a location situated at the Asian–North American interface....
Migration of waterfowl in the east asian flyway and spatial relationship to HPAI H5N1 outbreaks
John Y. Takekawa, S. H. Newman, X. Xiao, D.J. Prosser, K.A. Spragens, E.C. Palm, B. Yan, T. Li, F. Lei, D. Zhao, David C. Douglas, S.B. Muzaffar, W. Ji
2010, Avian Diseases (54) 466-476
Poyang Lake is situated within the East Asian Flyway, a migratory corridor for waterfowl that also encompasses Guangdong Province, China, the epicenter of highly pathogenic avian influenza (HPAI) H5N1. The lake is the largest freshwater body in China and a significant congregation site for waterfowl; however, surrounding rice fields and...
Validation of a real-time reverse transcriptase-PCR assay for the detection of H7 avian influenza virus
J. Pedersen, M.L. Killian, N. Hines, D. Senne, B. Panigrahy, Hon S. Ip, Erica Spackman
2010, Avian Diseases (54) 639-643
This report describes the validation of an avian influenza virus (AIV) H7 subtype-specific real-time reverse transcriptasePCR (rRT-PCR) assay developed at the Southeast Poultry Research Laboratory (SEPRL) for the detection of H7 AI in North and South American wild aquatic birds and poultry. The validation was a collaborative effort by the...
Intercontinental reassortment and genomic variation of low pathogenic avian influenza viruses isolated from northern pintails (Anas acuta) in Alaska: examining the evidence through space and time
Andrew M. Ramey, John M. Pearce, Paul L. Flint, S. Ip, Dirk V. Derksen, J. Christian Franson, Michael J. Petrula, Bradley D. Scotton, Kristine M. Sowl, Michael L. Wege, Kimberly A. Trust
2010, Virology (401) 179-189
Migration and population genetic data for northern pintails (Anas acuta) and phylogenetic analysis of low pathogenic avian influenza (LPAI) viruses from this host in Alaska suggest that northern pintails are involved in ongoing intercontinental transmission of avian influenza. Here, we further refine this conclusion through phylogenetic analyses which demonstrate that...
Migration of whooper swans and outbreaks of highly pathogenic avian influenza H5N1 virus in Eastern Asia
Scott H. Newman, Samuel A. Iverson, John Y. Takekawa, Martin Gilbert, Diann J. Prosser, Nyambyar Batbayar, Tseveenmyadag Natsagdorj, David C. Douglas
2009, PLoS ONE (4) 1-11
Evaluating the potential involvement of wild avifauna in the emergence of highly pathogenic avian influenza H5N1 (hereafter H5N1) requires detailed analyses of temporal and spatial relationships between wild bird movements and disease emergence. The death of wild swans (Cygnus spp.) has been the first indicator of the presence of H5N1...
Does influenza A affect body condition of wild mallard ducks, or vice versa?
Paul L. Flint, J. Christian Franson
2009, Proceedings of the Royal Society B: Biological Sciences (276) 2345-2346
Low pathogenic avian influenza (LPAI) viruses are well documented to circulate within wild waterfowl populations (Olsen et. al. 2006). It has been assumed that these infections are benign with no subsequent effects on life-history parameters. The study by Latorre-Margalef et al. (2009; hereafter L.-M. et al.) represents an important step,...
Avian influenza at both ends of a migratory flyway: characterizing viral genomic diversity to optimize surveillance plans for North America
John M. Pearce, Andrew M. Ramey, Paul L. Flint, Anson V. Koehler, Joseph P. Fleskes, J. Christian Franson, Jeffrey S. Hall, Dirk V. Derksen, S. Ip
2009, Evolutionary Applications (2) 457-468
Although continental populations of avian influenza viruses are genetically distinct, transcontinental reassortment in low pathogenic avian influenza (LPAI) viruses has been detected in migratory birds. Thus, genomic analyses of LPAI viruses could serve as an approach to prioritize species and regions targeted by North American surveillance activities for foreign origin...
Surveillance plan for the early detection of H5N1 highly pathogenic avian influenza virus in migratory birds in the United States: surveillance year 2009
Christopher J. Brand
2009, General Information Product 92
Executive Summary: This Surveillance Plan (Plan) describes plans for conducting surveillance of wild birds in the United States and its Territories and Freely-Associated States to provide for early detection of the introduction of the H5N1 Highly Pathogenic Avian Influenza (HPAI) subtype of the influenza A virus by migratory birds during...
Occurrence of viable avian influenza viruses in water and bed sediments from selected water bodies along the Atlantic Flyway, February and May 2006 and January 2007
Melinda S. Dalton, Lisa M. Stewart, S. Ip
2009, Open-File Report 2009-1161
Water and bed-sediment samples were collected from selected water bodies along the Atlantic Flyway and analyzed for the presence of viable avian influenza viruses. Samples were collected during February and May 2006 and January 2007 at U.S. Fish and Wildlife Service National Wildlife Refuges in Georgia, South Carolina, North Carolina,...
Breeding-season sympatry facilitates genetic exchange among allopatric wintering populations of Northern Pintails in Japan and California
Paul L. Flint, Kiyoaki Ozaki, John M. Pearce, Brian Guzzetti, Hiroyoshi Higuchi, Joseph P. Fleskes, Tetsuo Shimada, Dirk V. Derksen
2009, Condor (111) 591-598
The global redistribution of pathogens, such as highly pathogenic avian influenza, has renewed interest in the connectivity of continental populations of birds. Populations of the Northern Pintail (Anas acuta) wintering in Japan and California are considered separate from a management perspective. We used data from band recoveries and population genetics...
Experimental infection of a North American raptor, American kestrel (Falco sparverius), with highly pathogenic avian influenza virus (H5N1)
Jeffrey S. Hall, Hon S. Ip, J. C. Franson, C. Meteyer, Sean W. Nashold, Joshua L. Teslaa, J. French, P. Redig, C. Brand
2009, PLoS ONE (4)
Several species of wild raptors have been found in Eurasia infected with highly pathogenic avian influenza virus (HPAIV) subtype H5N1. Should HPAIV (H5N1) reach North America in migratory birds, species of raptors are at risk not only from environmental exposure, but also from consuming infected birds and carcasses. In this...
Surveillance for high pathogenicity avian influenza virus in wild birds in the Pacific Flyway of the United States, 2006-2007
Robert J. Dusek, J.B. Bortner, T.J. DeLiberto, J. Hoskins, J. Christian Franson, B.D. Bales, D. Yparraguirre, S.R. Swafford, Hon S. Ip
2009, Avian Diseases (53) 222-230
In 2006 the U.S. Department of Agriculture, U.S. Department of Interior, and cooperating state fish and wildlife agencies began surveillance for high-pathogenicity avian influenza (HPAI) H5N1 virus in wild birds in the Pacific Flyway of the United States. This surveillance effort was highly integrated in California, Oregon, Washington, Idaho, Nevada,...
Satellite-marked waterfowl reveal migratory connection between H5N1 outbreak areas in China and Mongolia
D.J. Prosser, John Y. Takekawa, S. H. Newman, B. Yan, David C. Douglas, Y. Hou, Z. Xing, Dongxiao Zhang, T. Li, Y. Li, D. Zhao, W.M. Perry, E.C. Palm
2009, Ibis (151) 568-576
The role of wild birds in the spread of highly pathogenic avian influenza H5N1 has been greatly debated and remains an unresolved question. However, analyses to determine involvement of wild birds have been hindered by the lack of basic information on their movements in central Asia. Thus, we initiated a...
Low prevalence of avian influenza virus in shorebirds on the Pacific coast of North America
Samuel A. Iverson, John Y. Takekawa, Steven Schwarzbach, Carol J. Cardona, Nils Warnock, Mary Anne Bishop, Greg A. Schirato, Sara Paroulek, Joshua T. Ackerman, S. Ip, Walter M. Boyce
2008, Waterbirds (31) 602-610
The emergence of highly pathogenic avian influenza (HPAI) H5N1 has elevated concerns about wild birds as virus hosts; however, little is known about the ecological and epidemiological factors of transmission by shorebirds. Here we summarize results for 2,773 shorebirds that were live-trapped on the Pacific coast of the United States...
[Book Review] Avian influenza
Steven Schwarzbach, Sabir Muzzafar, John Y. Takekawa
2008, Journal of Wildlife Diseases (44) 1056-1058
Review of: Avian Influenza. By David E. Swayne, Blackwell Publishing Ltd., Oxford, UK. 2008. 605 pp. ISBN-13 978-0-8138-2047-7/2008. US $149.99 (hardback)....
Duck migration and past influenza A (H5N1) outbreak areas
Nicolas Gaidet, Scott H. Newman, Ward Hagemeijer, Tim Dodman, Julien Cappelle, Saliha Hammoumi, Lorenzo De Simone, John Y. Takekawa
2008, Emerging Infectious Diseases (14) 1164-1166
In 2005 and 2006, the highly pathogenic avian influenza (HPAI) virus subtype H5N1 rapidly spread from Asia through Europe, the Middle East, and Africa. Waterbirds are considered the natural reservoir of low pathogenic avian influenza viruses (1), but their potential role in the spread of HPAI (H5N1), along with legal...
Evidence of infection by H5N2 highly pathogenic avian influenza viruses in healthy wild waterfowl
N. Gaidet, G. Cattoli, S. Hammoumi, S. H. Newman, W. Hagemeijer, John Y. Takekawa, J. Cappelle, T. Dodman, T. Joannis, P. Gil, I. Monne, A. Fusaro, I. Capua, S. Manu, P. Micheloni, U. Ottosson, J.H. Mshelbwala, J. Lubroth, J. Domenech, F. Monicat
2008, PLoS Pathogens (4)
The potential existence of a wild bird reservoir for highly pathogenic avian influenza (HPAI) has been recently questioned by the spread and the persisting circulation of H5N1 HPAI viruses, responsible for concurrent outbreaks in migratory and domestic birds over Asia, Europe, and Africa. During a large-scale surveillance programme over Eastern...
Seasonal movements and migration of Pallas's Gulls Larus ichthyaetus from Qinghai Lake, China
S.B. Muzaffar, John Y. Takekawa, D.J. Prosser, David C. Douglas, B. Yan, Z. Xing, Y. Hou, E.C. Palm, S. H. Newman
2008, Forktail (24) 100-107
We studied the seasonal movements and migration often Pallas's Gulls Larus ichthyaetus trom Qinghai Lake to assess migratory routes and stopover areas. Each individual was captured and equipped with an 18 g solar-powered Platform Transmitter Terminal (PIT) to track its movements from September 2007 to May 2008. Six...
Analytical validation of a real-time reverse transcription polymerase chain reaction test for Pan-American lineage H7 subtype Avian influenza viruses
Erica Spackman, Hon S. Ip, D.L. Suarez, R.D. Slemons, D.E. Stallknecht
2008, Journal of Veterinary Diagnostic Investigation (20) 612-616
A real-time reverse transcription polymerase chain reaction test for the identification of the H7 subtype in North American Avian influenza viruses (AIVs) was first reported in 2002; however, recent AIV surveillance efforts in wild birds and H7 outbreaks in poultry demonstrated that the 2002 test did not detect all H7...
A blood survey of elements, viral antibodies, and hemoparasites in wintering Harlequin Ducks (Histrionicus histrionicus) and Barrow's Goldeneyes (Bucephala islandica)
D.J. Heard, D.M. Mulcahy, S. A. Iverson, D.J. Rizzolo, E.C. Greiner, J. Hall, S. Ip, Daniel Esler
2008, Journal of Wildlife Diseases (44) 486-493
Twenty-eight Harlequin Ducks (Histrionicus histrionicus) and 26 Barrow's Goldeneyes (Bucephala islandica) were captured in Prince William Sound, Alaska, between 1 and 15 March 2005. Blood was collected for quantification of element concentrations, prevalence of antibodies to several viruses, and hemoparasite prevalence and identification. Although we found selenium concentrations that have...