USGS

Biodegradation of Chlorinated Ethenes at a Karst Site in Middle Tennessee

Table 12. Aerobic and facultative anaerobic heterotrophic bacteria enumeration data


[mL, milliliters; CFU, colony-forming units; CFU/100mL, colony-forming units per 100 milliliters; TNTC, too numerous to count; >, greater than]

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                                                                                   Enumeration results
                                  Replicate  Incubation	   Source of water    _____________________________
    Microcosm description	    number     (weeks)     (date collected)   mL of sample  CFU	 CFU/100 mL
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Experiment 1, treatment 9 	      1	          1	   2D/12D (2/17/98)	  1.00	   TNTC	  > 2 x 104	
Experiment 1, treatment 9 	      2	          1	   2D/12D (2/17/98)	  1.00	   TNTC	  > 2 x 104	
Experiment 1, treatment 9 	      3	          1	   2D/12D (2/17/98)	  1.00	   TNTC	  > 2 x 104	
Experiment 1, treatment 9 	      4	          1	   2D/12D (2/17/98)	  1.00	   TNTC	  > 2 x 104	
Experiment 2, treatment 1 	      2	          3	     1D (5/21/98)	  0.01	      6	    6 x 104	

Experiment 2, treatment 1 	      4	          3	     1D (5/21/98)	  0.01	     89	  8.9 x 105	
Experiment 2, treatment 2 	      3	          3	     2D (5/21/98)	  0.01	   TNTC   > 2 x 106	
Experiment 2, treatment 2 	      4	          3	     2D (5/21/98)	  0.01	   TNTC	  > 2 x 106	
Experiment 2, treatment 3 	      3	          3	     3D (5/21/98)	  0.01	    134	  1.3 x 106	
Experiment 2, treatment 3 	      4	          3	     3D (5/21/98)	  0.01	   TNTC	  > 2 x 106	

Experiment 2, treatment 4 	      3	          3	     12D (5/21/98)	  0.01	    121	  1.2 x 106	
Experiment 2, treatment 4 	      4	          3	     12D (5/21/98)	  0.01	     86	  8.6 x 105	
Experiment 2, treatment 5 (control)   1	          3	    2D/3D (5/21/98)	  1.00	      0	          0	
Experiment 2, treatment 5 (control)   2	          3	    2D/3D (5/21/98)	  1.00	      0	          0	
Experiment 2, treatment 5 (control)   3	          3	    2D/3D (5/21/98)	  1.00	      0	          0

Experiment 2, treatment 5 (control)   4	          3	    2D/3D (5/21/98)	  1.00	      0	          0	
Experiment 2, treatment 1 	      1	         17	     1D (5/21/98)	  0.01	   TNTC	  > 2 x 106	
Experiment 2, treatment 1 	      4	         17	     1D (5/21/98)	  0.01	   TNTC	  > 2 x 106	
Experiment 2, treatment 2 	      1	         17	     2D (5/21/98)	  0.01	   TNTC   > 2 x 106	
Experiment 2, treatment 2 	      4	         17	     2D (5/21/98)	  0.01	   TNTC	  > 2 x 106	

Experiment 2, treatment 3 	      1  	 17	     3D (5/21/98)	  0.01	   TNTC	  > 2 x 106	
Experiment 2, treatment 3 	      1	         17	     3D (5/21/98)	  0.01	   TNTC	  > 2 x 106	
Experiment 2, treatment 4 	      4	         17	     12D (5/21/98)	  0.01	   TNTC	  > 2 x 106	
Experiment 2, treatment 4 	      4	         17	     12D (5/21/98)	  0.01	   TNTC	  > 2 x 106	
Experiment 2, treatment 5 (control)   1	         17	    2D/3D (5/21/98)	  0.01	      0	          0	

Experiment 2, treatment 5 (control)   2	         17	    2D/3D (5/21/98)	  0.01	      0	          0	
Experiment 2, treatment 5 (control)   3	         17	    2D/3D (5/21/98)	  0.01	      0	          0	
Experiment 2, treatment 5 (control)   4	         17	    2D/3D (5/21/98)	  0.01	      0	          0	
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