Coarse Fraction
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Fine Fraction
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transfer the sediment from the beaker and gently re-sieve through a set of nested 2 phi and 4 phi sieves using filtered sea water and add the wash to the fine fraction in the mason jar
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Determine sieved volume using a graduated cylinder, Record and return solution to the mason jar
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examine the sediment retained on each sieve using a light microscope; characterize particles as to detrital,aggregates, fecal pellets, etc. and, qualitatively, determine relative percentages of each.
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transfer contents from each sieve to a 100 ml glass beaker with filtered seawater and sonify in a sonic bath for 30 minutes
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Homogenize solution with a stir bar and extract 10-20 ml with a pipette; transfer to a millipore rig using a pre-weighed 47 mm diameter/0.4 um nucleopore filter; filter solution and rinse five times with fdw-record both volumes
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re-sieve contents through a 4 phi sieve using a 200 ml tall-boy beaker as a catch pan.....................
Return sediment retained on 4phi sieve for standard size analysis
using whole phi nested sieves (include -1 phi fraction as well)
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dry filter overnight under a laminar flow hood; determine weight of sediment (g)- this is weight of fines in the (silts-clays) sample
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resuspend the filtrate with a motorized stirrer and transfer an aliquot of sieved solution with a pipette and filter through a standard millipore filtration rig using a pre-weighed 47 mm diameter/0.4 um nucleopore filter; rinse filter and sediment 5 times with filter distilled water and record volume of aliquot filtered and final volume
collected in the flask with a graduated cylinder;
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analyze the solution in the
mason jar by standard coulter
counter methods- this will
determine the distribution
of silt and clay sized particles
within the sample |
transfer filter to a petrie dish and dry overnight under a laminar flow hood; record weight of filter and determine weight of sediment normalized to grams. This is an operationally defined measure of the weight of aggregates within the sample. |
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