Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci
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Abstract
Canada goose (Branta Canadensis) and harlequin duck (Histrionicus histrionicus) DNAs were digested with Sau3AI, and size selected (300-700 bp) fragments were ligated into BamHI-digested pBluscriptII KS+. The enrichment protocol of Ostrander et al.1 was followed. The resulting libraries were screened using a [ƴ-32P]ATP end-labelled (CA)20 oligonucleotides as a hybridization probe. Positive clones were sequenced using cycle-sequencing protocols (Epicentre Technologies, Madison, WI) and primers flanking the inserts. PCR primers were designed to amplify the repeat and yield amplification products of ≈100-200 bp. DNA samples were screened for variation at these loci using [ƴ-32P]ATP end-labelled primers. The products were resolved using 6% denaturing polyacrylamide gels and autoradiography.
Suggested Citation
Buchholz, W., Pearce, J.M., Pierson, B.J., and Scribner, K.T., 1998, Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci: Animal Genetics, v. 29, no. 4, p. 323-325, https://doi.org/10.1046/j.1365-2052.1998.00247.x.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci |
| Series title | Animal Genetics |
| DOI | 10.1046/j.1365-2052.1998.00247.x |
| Volume | 29 |
| Issue | 4 |
| Publication Date | August 11, 2003 |
| Year Published | 1998 |
| Language | English |
| Publisher | Wiley |
| Contributing office(s) | Alaska Biological Science Center, Alaska Science Center |
| Description | 2 p. |
| First page | 323 |
| Last page | 325 |