Induction of antibody-producing cells in rainbow trout, Salmo gairdneri Richardson, by flush exposure
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Abstract
Splenic antibody-producing cells were produced by rainbow trout that had been exposed to O-antigens extracted from Yersinia ruckeri and Aeromonas salmonicida by adding the concentrated antigen preparation directly into the water of the tank holding the fish for a flush exposure. This method was compared with the proven techniques of exposure: intraperitoneal injection or a 2 minute immersion of the fish in the antigen preparation. Dosage experiments showed that the production of antibody-producing cells was induced by the immersion of trout for 2 minutes in water with 5.0 μg/ml-1 (or more) with the Y. ruckeri O-antigen, or 500 μg ml-1 (or more) of the A. salmonicida O-antigen. Similar differences were evident when the respective antigens were added directly to the water.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Induction of antibody-producing cells in rainbow trout, Salmo gairdneri Richardson, by flush exposure |
| Series title | Journal of Fish Biology |
| DOI | 10.1111/j.1095-8649.1979.tb03611.x |
| Volume | 15 |
| Issue | 3 |
| Publication Date | January 24, 2006 |
| Year Published | 1979 |
| Language | English |
| Publisher | Wiley |
| Contributing office(s) | Leetown Science Center |
| Description | 6 p. |
| First page | 317 |
| Last page | 322 |