Plaque-forming cells and humoral antibody in rainbow trout (Salmo gairdneri) induced by immersion in a Yersinia ruckeri O-antigen preparation
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Abstract
Rainbow trout (Salmo gairdneri) were exposed to the O-antigen of Yersinia ruckeri by various immunization regimens. The passive hemolytic plaque assay was used to show specific splenic plaque-forming cells (PFC) and passive hemagglutination demonstrated humoral antibody titers in fish injected with or immersed in the antigen preparations. Preceding antigen immersion with a 2-min immersion in either a 5.2 or a 2.6% NaCl solution did not affect the numbers of PFC or levels of antibody. Fish held for 2 min in ethyl m-aminobenzoate methanesulfonate (MS-222®) before antigen immersion showed no significant difference from the control fish. Dosage studies showed that the minimal concentration of antigen for induction of PFC by immersion was 5.0 μg/mL.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Plaque-forming cells and humoral antibody in rainbow trout (Salmo gairdneri) induced by immersion in a Yersinia ruckeri O-antigen preparation |
| Series title | Journal of the Fisheries Research Board of Canada |
| DOI | 10.1139/f79-092 |
| Volume | 36 |
| Issue | 6 |
| Year Published | 1979 |
| Language | English |
| Publisher | Canadian Science Publishing |
| Contributing office(s) | Leetown Science Center |
| Description | 4 p. |
| First page | 636 |
| Last page | 639 |