Experimental propagation of lymphocystis disease of fishes
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Abstract
The agent of lymphocystis disease has been propagated under laboratory conditions for more than 2 years. Although several hatchery-propagated centrarchids are susceptible, the bluegill (Lepomis macrochirus) is the host of choice because it is most easily maintained. At 12.5° the disease was transmitted by implantation and by injection, but there was no evidence of fish-to-fish spread, nor seasonal change in host susceptibility. The infective agent was clearly demonstrated to be filterable (Millipore HA). It retained infectivity in storage at −20° for almost two years, and its viability after desiccation was confirmed. These facts support its postulated viral nature. The virus was glycerol- and ether-sensitive and did not induce formation of giant lymphocystis cells in monolayer cultures of bluegill ovary cells.
Suggested Citation
Wolf, K., 1962, Experimental propagation of lymphocystis disease of fishes: Virology, v. 18, no. 2, p. 249-256, https://doi.org/10.1016/0042-6822(62)90011-9.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Experimental propagation of lymphocystis disease of fishes |
| Series title | Virology |
| DOI | 10.1016/0042-6822(62)90011-9 |
| Volume | 18 |
| Issue | 2 |
| Year Published | 1962 |
| Language | English |
| Publisher | Elsevier |
| Contributing office(s) | Leetown Science Center |
| Description | 8 p. |
| First page | 249 |
| Last page | 256 |