Molecular and genetic characterization of cytochrome oxidase negative Aeromonas salmonicida isolated from coho salmon (Oncorhynchus kisutch)
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Abstract
Cytochrome oxidase variants of the bacterial fish pathogen, Aeromonas salmonicida, were characterized for genetic and molecular homology with cytochrome oxidase-positive isolates that typically induce furunculosis in salmonids. Protein and lipopolysaccharide moieties of the cytochrome oxidase-negative variants were similar to their typical counterparts, based on sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. Pathogenicity of aberrant isolates to brook trout (Salvelinus fontinalis) was similar to typical cytochrome oxidase-positive isolates. Colorimetric deoxyribonucleic acid (DNA) hybridization in 96-well microplates yielded homology values greater than 82.5% for typical aberrant A. salmonicida isolates when photobiotinylated DNA for reference A. salmonicida 3.101 was used as a probe. The only variation of these isolates from typical A. salmonicida was a negative cytochrome oxidase reaction.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Molecular and genetic characterization of cytochrome oxidase negative Aeromonas salmonicida isolated from coho salmon (Oncorhynchus kisutch) |
| Series title | Journal of Wildlife Diseases |
| DOI | 10.7589/0090-3558-28.4.515 |
| Volume | 28 |
| Issue | 4 |
| Year Published | 1992 |
| Language | English |
| Publisher | Wildlife Disease Association |
| Contributing office(s) | Leetown Science Center |
| Description | 6 p. |
| First page | 515 |
| Last page | 520 |
| Google Analytic Metrics | Metrics page |