Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters
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Abstract
To improve surveillance for Cryptosporidium oocysts in water, the US Environmental Protection Agency developed method 1622, which consists of filtration, concentration, immunomagnetic separation, fluorescent antibody and 4, 6-diamidino-2-phenylindole (DAPI) counter-staining, and microscopic evaluation. Two filters were compared for analysis of 11 stream water samples collected throughout the United States. Replicate 10-L stream water samples (unspiked and spiked with 100–250 oocysts) were tested to evaluate matrix effects. Oocyst recoveries from the stream water samples averaged 22% (standard deviation [SD] = ±17%) with a membrane disk and 12% (SD = ±6%) with a capsule filter. Oocyst recoveries from reagent water precision and recovery samples averaged 39% (SD = ±13%) with a membrane disk and 47% (SD = ±19%) with a capsule filter. These results demonstrate that Cryptosporidium oocysts can be recovered from stream waters using method 1622, but recoveries are lower than those from reagent-grade water. This research also evaluated concentrations of indicator bacteria in the stream water samples. Because few samples were oocyst-positive, relationships between detections of oocysts and concentrations of indicator organisms could not be determined.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters |
| Series title | Journal - American Water Works Association |
| DOI | 10.1002/j.1551-8833.2001.tb09101.x |
| Volume | 93 |
| Issue | 1 |
| Year Published | 2001 |
| Language | English |
| Publisher | American Water Works Association |
| Publisher location | Denver, CO, United States |
| Description | 10 p. |
| First page | 78 |
| Last page | 87 |
| Google Analytic Metrics | Metrics page |