Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy

Analyst
By: , and 

Links

Abstract

FTIR and Raman spectromicroscopy were used to characterize the composition of Curvularia protuberata hyphae, and to compare a strain isolated from plants inhabiting geothermal soils with a non-geothermal isolate. Thermal IR source images of hyphae have been acquired with a 64 × 64 element focal plane array detector; single point IR spectra have been obtained with synchrotron source light. In some C. protuberata hyphae, we have discovered the spectral signature of crystalline mannitol, a fungal polyol with complex protective roles. With FTIR-FPA imaging, we have determined that the protein content in cells remains fairly constant throughout the length of a hypha, whereas the mannitol is found at discrete, irregular locations. This is the first direct observation of mannitol in intact fungal hyphae. Since the concentration of mannitol in cells varies with respect to position and is not present in all hyphae, this discovery may be related to habitat adaptation, fungal structure and growth stages.

Publication type Article
Publication Subtype Journal Article
Title Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy
Series title Analyst
DOI 10.1039/c0an00534g
Volume 135
Issue 12
Year Published 2010
Language English
Publisher Royal Society of Chemistry
Contributing office(s) Western Fisheries Research Center
Description 6 p.
First page 3249
Last page 3254
Google Analytic Metrics Metrics page
Additional publication details