Development and characterization of 16 polymorphic microsatellite loci for the Alaska blackfish (Esociformes: Dallia pectoralis)
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Abstract
Blackfishes (Esociformes: Esocidae: Dallia), small fishes with relictual distributions, are unique in being the only primary freshwater fish genus endemic to Beringia. Although the number of species of Dallia is debated, disjunct populations and distinct mitochondrial divisions that predate the end of the last glacial maximum are apparent. We developed sixteen polymorphic microsatellites from the Alaska blackfish (Dallia pectoralis) to study genetic diversity in Dallia. Genotypes from two populations, Denali (n = 31) and Bethel (n = 35), demonstrated the usefulness of the loci for population-level investigation. Observed and expected heterozygosity averaged 18.6 and 19.8 % in Denali and 61.1 and 63.7 % in Bethel. Number of alleles at each locus averaged 3.50 in Denali and 9.63 in Bethel. The observed signature of variability and structuring between populations is consistent with mitochondrial data.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Development and characterization of 16 polymorphic microsatellite loci for the Alaska blackfish (Esociformes: Dallia pectoralis) |
| Series title | Conservation Genetics Resources |
| DOI | 10.1007/s12686-013-0091-6 |
| Volume | 6 |
| Issue | 2 |
| Year Published | 2014 |
| Language | English |
| Publisher | Springer |
| Contributing office(s) | Alaska Science Center |
| Description | 3 p. |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Conservation Genetics Resources |
| First page | 349 |
| Last page | 351 |
| Google Analytic Metrics | Metrics page |