Molecular cloning and sequence analysis of the gene coding for the 57kDa soluble antigen of the salmonid fish pathogen Renibacterium salmoninarum
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Abstract
The complete sequence coding for the 57-kDa major soluble antigen of the salmonid fish pathogen, Renibacterium salmoninarum, was determined. The gene contained an opening reading frame of 1671 nucleotides coding for a protein of 557 amino acids with a calculated Mr value of 57190. The first 26 amino acids constituted a signal peptide. The deduced sequence for amino acid residues 27–61 was in agreement with the 35 N-terminal amino acid residues determined by microsequencing, suggesting the protein in synthesized as a 557-amino acid precursor and processed to produce a mature protein of Mr 54505. Two regions of the protein contained imperfect direct repeats. The first region contained two copies of an 81-residue repeat, the second contained five copies of an unrelated 25-residue repeat. Also, a perfect inverted repeat (including three in-frame UAA stop codons) was observed at the carboxyl-terminus of the gene.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Molecular cloning and sequence analysis of the gene coding for the 57kDa soluble antigen of the salmonid fish pathogen Renibacterium salmoninarum |
| Series title | FEMS Microbiology Letters |
| DOI | 10.1111/j.1574-6968.1992.tb05427.x |
| Volume | 96 |
| Issue | 2-3 |
| Year Published | 1992 |
| Language | English |
| Publisher | Wiley |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 8 p. |
| First page | 259 |
| Last page | 266 |
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