Molecular cloning and sequence analysis of the gene coding for the 57kDa soluble antigen of the salmonid fish pathogen Renibacterium salmoninarum
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Abstract
The complete sequence coding for the 57-kDa major soluble antigen of the salmonid fish pathogen, Renibacterium salmoninarum, was determined. The gene contained an opening reading frame of 1671 nucleotides coding for a protein of 557 amino acids with a calculated Mr value of 57190. The first 26 amino acids constituted a signal peptide. The deduced sequence for amino acid residues 27–61 was in agreement with the 35 N-terminal amino acid residues determined by microsequencing, suggesting the protein in synthesized as a 557-amino acid precursor and processed to produce a mature protein of Mr 54505. Two regions of the protein contained imperfect direct repeats. The first region contained two copies of an 81-residue repeat, the second contained five copies of an unrelated 25-residue repeat. Also, a perfect inverted repeat (including three in-frame UAA stop codons) was observed at the carboxyl-terminus of the gene.
Suggested Citation
Chien, M., Gilbert, T.L., Huang, C., Landolt, M.L., O’Hara, P.J., Winton, J.R., 1992, Molecular cloning and sequence analysis of the gene coding for the 57kDa soluble antigen of the salmonid fish pathogen Renibacterium salmoninarum: FEMS Microbiology Letters, v. 96, no. 2-3, p. 259-266, https://doi.org/10.1111/j.1574-6968.1992.tb05427.x.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Molecular cloning and sequence analysis of the gene coding for the 57kDa soluble antigen of the salmonid fish pathogen Renibacterium salmoninarum |
| Series title | FEMS Microbiology Letters |
| DOI | 10.1111/j.1574-6968.1992.tb05427.x |
| Volume | 96 |
| Issue | 2-3 |
| Year Published | 1992 |
| Language | English |
| Publisher | Wiley |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 8 p. |
| First page | 259 |
| Last page | 266 |