Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola
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Abstract
The fountain darter Etheostoma fonticola (FOD) is a federally endangered fish listed under the US Endangered Species Act. Here, we identified and characterized a novel aquareovirus isolated from wild fountain darters inhabiting the San Marcos River. This virus was propagated in Chinook salmon embryo (CHSE)-214, rainbow trout gonad-2 and fathead minnow cells at 15°C. The epithelioma papulosum cyprini cell line was refractory at all temperatures evaluated. High throughput sequencing technologies facilitated the complete genome sequencing of this virus utilizing ribosomal RNA-depleted RNA extracted from infected CHSE-214 cells. Conventional PCR primer sets were developed for the detection and confirmation of this virus to assist diagnostic screening methods. Phylogenetic analysis suggests this virus belongs to the Aquareovirus A genus. This research provides requisite initial data critical to support hatchery and refugia biosecurity measures for this endangered species.
Suggested Citation
Iwanowicz, L., Iwanowicz, D., Adams, C.R., Lewis, T., Brandt, T., Sanders, L.R., and Cornman, R.S., 2019, Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola: Diseases of Aquatic Organisms, v. 130, no. 2, p. 95-108, https://doi.org/10.3354/dao03261.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Isolation, characterization and molecular identification of a novel aquareovirus that infects the endangered fountain darter, Etheostoma fonticola |
| Series title | Diseases of Aquatic Organisms |
| DOI | 10.3354/dao03261 |
| Volume | 130 |
| Issue | 2 |
| Year Published | 2019 |
| Language | English |
| Publisher | Inter-Research |
| Contributing office(s) | Fort Collins Science Center, Leetown Science Center |
| Description | 14 p. |
| First page | 95 |
| Last page | 108 |