Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly, Lednia tumana, in environmental samples
Conservation Genetics Resources
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Abstract
A probe-based quantitative real-time PCR assay was developed to detect meltwater stonefly (Lednia tumana) environmental (e)DNA in water samples. The limits of detection and quantification, respectively, were 12.1 and 58.4 gene copies for calibration standards and these values were similarly low in a relevant environmental sample matrix (8.6 and 174.2, respectively). The assay’s utility was demonstrated in situ on water samples with concomitant manual invertebrate surveys from a wide range of alpine streams across L. tumana’s native range.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly, Lednia tumana, in environmental samples |
| Series title | Conservation Genetics Resources |
| DOI | 10.1007/s12686-023-01307-5 |
| Volume | 15 |
| Year Published | 2023 |
| Language | English |
| Publisher | Springer |
| Contributing office(s) | Northern Rocky Mountain Science Center |
| Description | 3 p. |
| First page | 105 |
| Last page | 107 |
| Google Analytic Metrics | Metrics page |