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Open-File Report 2015–1104

Exposure-Related Effects of Pseudomonas fluorescens, Strain CL145A, on Coldwater, Coolwater, and Warmwater Fish

By James A. Luoma, Kerry L. Weber, and Denise A. Mayer

Thumbnail of and link to report PDF (56.6 MB)Abstract

The exposure-related effects of a commercially prepared spray-dried powder (SDP) formulation of Pseudomonas fluorescens, strain CL145A, were evaluated on coldwater, coolwater, and warmwater fish endemic to the Great Lakes and Upper Mississippi River Basins. Nine species of young-of-the-year fish were exposed to SDP for 24 hours by using continuous-flow, serial-dilution exposure systems at temperatures of 12 degrees Celsius (°C; 2 species; Oncorhynchus mykiss [rainbow trout] and Salvelinus fontinalis [brook trout]), 17 °C (3 species; Perca flavescens [yellow perch], Sander vitreus [walleye], and Acipenser fulvescens [lake sturgeon]), or 22 °C (4 species; Micropterus salmoides [largemouth bass], Micropterus dolomieu [smallmouth bass], Lepomis macrochirus [bluegill sunfish], and Ictalurus punctatus [channel catfish]).

Treatments, which were nominal target concentrations of SDP (as active ingredient) of 50, 100, 200, and 300 milligrams per liter (mg/L), were continuously applied for 24 hours by the addition of a test article stock solution into the main water inflow of each exposure system’s dilution box. The SDP-treated water was then serially diluted through a series of dilution cells before delivery to the test chambers. The exposure concentrations measured were 61.5 to 81.4 percent of the target concentration. After exposure, fish were monitored for 22 days to assess exposure-related latent effects.

Analyses of test animal condition factors and survival revealed that a 24-hour continuous dose of SDP affected all species. Calculated concentrations of SDP that would be lethal to 50 percent of the test animals (LC50) for the coldwater species were 19.2 and 104.6 mg/L for rainbow and brook trout, respectively. The LC50’s for the coolwater species were 185.4, 176.9 and 8.9 mg/L for yellow perch, walleye, and lake sturgeon, respectively. The LC50’s for the warmwater species were 173.6, 139.4, and 63.1 for the largemouth bass, smallmouth bass, and channel catfish, respectively. A reliable LC50 for bluegill sunfish could not be calculated because mortality in the SDP-treated groups did not exceed 20 percent.

Further investigations to evaluate the SDP-exposure related effects on freshwater fish at the maximum approved open-water label concentration and exposure duration (100 mg/L for 8 hours) and using the expected lentic application technique (static application) are warranted. The variation in tolerance to P. fluorescens, strain CL145A, exposure observed in this study indicates that fish species community composition should be considered before SDP is applied in open-water environments.

First posted May 22, 2015

  • Open-File Report 2015-1050
    Companion Report - Efficacy of Pseudomonas fluorescens (Pf-CL145A) Spray Dried Powder for Controlling Zebra Mussels Adhering to Test Substrates
  • Open-File Report 2015-1051
    Companion Report - Efficacy of Pseudomonas fluorescens Strain CL145A Spray Dried Powder for Controlling Zebra Mussels Adhering to Native Unionid Mussels Within Field Enclosures
  • Open-File Report 2015-1064
    Companion Report - Safety of Spray-Dried Powder Formulated Pseudomonas fluorescens Strain CL145A Exposure to Subadult/Adult Unionid Mussels During Simulated Open-Water Treatments
  • Open-File Report 2015-1066
    Companion Report - Exposure-Related Effects of Pseudomonas fluorescens (Pf-CL145A) on Juvenile Unionid Mussels
  • Open-File Report 2015-1094
    Companion Report - Exposure-Related Effects of Formulated Pseudomonas fluorescens Strain CL145A to Glochidia from Seven Unionid Mussel Species

For additional information, contact:
Director, Upper Midwest Environmental Sciences Center
U.S. Geological Survey
2630 Fanta Reed Road
La Crosse, WI 54603

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Suggested citation:

Luoma, J.A., Weber, K.L., and Mayer, D.A., 2015, Exposure-related effects of Pseudomonas fluorescens, strain CL145A, on coldwater, coolwater, and warmwater fish: U.S. Geological Survey Open-File Report 2015–1104, 1632 p.,

ISSN 2331-1258 (online)





Materials and Methods

Experimental Design

Data Analysis

Results and Discussion


References Cited

Appendix 1. Study Protocol, Amendments, and Datasheets

Appendix 2. Deviations From the Study Protocol

Appendix 3. Randomization Assignments

Appendix 4. Test Article Information

Appendix 5. Test Animal Information

Appendix 6. Test Animal Feed Information

Appendix 7. Water Quality

Appendix 8. Spectrophotometric Summary, SAS Outputs, Programs, and Logs

Appendix 9. Condition Index and Survival Assessment Summaries, SAS Outputs, Programs, and Logs

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